Home/ Questions/Which of the following is the best method to sterilize heat-labile solutions?
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kttutoria
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Asked: 2022-04-07T15:11:45+00:00 In:

Which of the following is the best method to sterilize heat-labile solutions?

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Question 1. Which of the following is the BEST technique to sterilize heat-labile solutions?

  • A. Membrane filtration.
  • B. Pasteurization.
  • C. Autoclave.
  • D. Dry heat.

Question 2: Pasteur’s Swan-necked experiment supports that:

  • A. Microbes require nutrients to grow.
  • B. The air is free from microbes.
  • C. Microbes are present in air.
  • D. Microbes emerge spontaneously.

Question 3: Which of the following techniques is NOT suitable for long-term storage of microorganisms?

  • A. Cryopreservation.
  • B. Freezing in liquid nitrogen.
  • C. Periodic transfer to fresh medium.
  • D. Freeze-drying.

♦ Relevant knowledge
Sterilization is the process of killing bacteria and microorganisms that adhere to the surface of instruments, laboratory equipment, etc. Harmful agents can be mentioned such as fungi, microorganisms, bacteria, animals… The effectiveness of the sterilization process depends heavily on the sterilized surface, the degree of bacterial contamination and the quantity. 

Common sterilization methods

1. Steam sterilization: Microorganisms that are exposed to saturated steam at high pressure will cause them to be completely destroyed due to the denaturation of enzyme components and molecular protein structure.

2. Dry heat method: In the process of sterilization by dry heat is also considered the oxidation of cellular components. This requires a higher temperature than a humid one and a longer exposure time.

3. Filtration method: This method is used mainly for non-thermal test solutions.

4. Ionizing radiation method

5. Gas method  

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1 Answer

  1. Best Answer
    Expert

    The correct answer:

    Q1. A-Membrane filtration.

    Q2. C-Microbes are present in air.

    Q3. C-Periodic transfer to fresh medium.

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